In determination and evaluation of pitiutary gonadotropin,. complicated biological methods for the assessment have been employed. However, recent advancement has clarified the physical ´,and chemical nature of the substance: In cope with this advancement, a new quantitative analytical method, . Crooke´s method, has been adapted enabling clinical use with the omission of complicacy in the procedure.
Furthermore, this procedure has provided fractional analysis of gonado-tropin into GA and- GB which correspond to FSH and LH respectively, and the knowledge of interrelationship aid of balance between these fractions in various endocrinological environments.
It has been well known that this procedure paved the way to establishment of diagnosis and therapeutic measures in case of functional disorders involving hypothalamus-pituitary-ovary system.
By this chemical method, the levels of urinary gonadotropin in normal menstrual cycles and various irregular menstrual cycles were measured and the comparison between them were made.
So called "feed back mechanism" upon hypothalamus-pituitary system, yet inconclusive, were reviewed from the view point of chage in urinary gonadotropin excretion following the administration of estrogen benzoate.
The resuts are as follows.
1) The presence of gonadotrooin in -female urine obtained from the individuals with regularity of catamenial cycles and the -highest level of gonadotropin were observed during the ovulation time. The average levels in, urine were GA. 197r/24hrs. and. GB,80r/24hrs. GA/GB was 2.5.
2) In comparison with the level of GA. GB, and GA/GB in normal cycles, the amount of GA and GB, also their ratio in patient with abnormal menstrual cycles difference. The levels of GB were especially deviated from normal ranges.
3) The adminaistration of estrogen benzoate were likely,to be influential to the level´ of urinary gonadotropin in abnormally menstruating individuals, especially to GB. In case the estrogenic hormone 10,000 units (I.U.) given daily, increase of GA and GB were observed. On the contrary, administration of a single dose of more than 50,000 units of estrogen showed inhibitory effects. The amount of estrogenic hormone which acted as inhibitor, if given as single dose, would increase the amount of GA and GB in case of the same amount divided.
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